› 1cet

X-ray diffraction
2.05Å resolution

CHLOROQUINE BINDS IN THE COFACTOR BINDING SITE OF PLASMODIUM FALCIPARUM LACTATE DEHYDROGENASE.

Released:
DOI: 10.2210/pdb1cet/pdb
PDB entry 1cet coloured by chain, front view.
PDB entry 1cet coloured by chain, side view.
PDB entry 1cet coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Chloroquine binds in the cofactor binding site of Plasmodium falciparum lactate dehydrogenase.
Read JA, Wilkinson KW, Tranter R, Sessions RB, Brady RL
J Biol Chem 274 10213-8 (1999)
PMID: 10187806

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.16 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27743 (Residues: 1-316; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CLQ 1 x CLQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: I222
Unit cell:
a: 79.93Å b: 85.44Å c: 92.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.154 0.193
Expression system: Escherichia coli

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Citations

4 review citations

Potential biomarkers and their applications for rapid and reliable detection of malaria.
Jain et al. (2014)
3 more

12 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
11 more