› 1cf7

X-ray diffraction
2.6Å resolution

STRUCTURAL BASIS OF DNA RECOGNITION BY THE HETERODIMERIC CELL CYCLE TRANSCRIPTION FACTOR E2F-DP

Released:
DOI: 10.2210/pdb1cf7/pdb
PDB entry 1cf7 coloured by chain, front view.
PDB entry 1cf7 coloured by chain, side view.
PDB entry 1cf7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of DNA recognition by the heterodimeric cell cycle transcription factor E2F-DP.
Zheng N, Fraenkel E, Pabo CO, Pavletich NP
Genes Dev 13 666-74 (1999)
PMID: 10090723

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113538 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor E2F4 Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16254 (Residues: 11-86; Coverage: 18%)
Gene name: E2F4
Sequence domains: E2F/DP family winged-helix DNA-binding domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Transcription factor Dp-2 Chain: B
Molecule details ›
Chain: B
Length: 95 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14188 (Residues: 121-215; Coverage: 21%)
Gene names: DP2, TFDP2
Sequence domains: E2F/DP family winged-helix DNA-binding domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*TP*TP*TP*TP*CP*GP*CP*GP*CP*GP*GP*TP*TP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
DNA (5'-D(*TP*AP*AP*AP*AP*CP*CP*GP*CP*GP*CP*GP*AP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.91 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3121
Unit cell:
a: 101.3Å b: 101.3Å c: 73.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 not available
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Regulation of the G2/M transition by p53.
Taylor et al. (2001)
18 more

31 mentions without citation

Atypical E2Fs: new players in the E2F transcription factor family.
Lammens et al. (2009)
30 more