› 1cgu

X-ray diffraction
2.5Å resolution

CATALYTIC CENTER OF CYCLODEXTRIN GLYCOSYLTRANSFERASE DERIVED FROM X-RAY STRUCTURE ANALYSIS COMBINED WITH SITE-DIRECTED MUTAGENESIS

Released:
DOI: 10.2210/pdb1cgu/pdb
PDB entry 1cgu coloured by chain, front view.
PDB entry 1cgu coloured by chain, side view.
PDB entry 1cgu coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Catalytic center of cyclodextrin glycosyltransferase derived from X-ray structure analysis combined with site-directed mutagenesis.
Klein C, Hollender J, Bender H, Schulz GE
Biochemistry 31 8740-6 (1992)
PMID: 1390660

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 684 amino acids
Theoretical weight: 74.52 KDa
Source organism: Niallia circulans
Expression system: Not provided
UniProt:
  • Canonical: P30920 (Residues: 35-718; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 94.1Å b: 105Å c: 113.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 not available
Expression system: Not provided

Quick links

Citations

13 review citations

Mechanisms of enzymatic glycoside hydrolysis.
McCarter et al. (1994)
12 more

2 mentions without citation

Defining structural and evolutionary modules in proteins: a community detection approach to explore sub-domain architecture.
Hleap et al. (2013)
1 more