› 1cip

X-ray diffraction
1.5Å resolution

GI-ALPHA-1 SUBUNIT OF GUANINE NUCLEOTIDE-BINDING PROTEIN COMPLEXED WITH A GTP ANALOGUE

Released:
DOI: 10.2210/pdb1cip/pdb
PDB entry 1cip coloured by chain, front view.
PDB entry 1cip coloured by chain, side view.
PDB entry 1cip coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of Gialpha1.GppNHp, autoinhibition in a galpha protein-substrate complex.
Coleman DE, Sprang SR
J Biol Chem 274 16669-72 (1999)
PMID: 10358003

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.27 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 2-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1, CHESS BEAMLINE F1
Spacegroup: P3221
Unit cell:
a: 80.05Å b: 80.05Å c: 105.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.235
Expression system: Escherichia coli

Quick links

Citations

7 review citations

GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins.
Ross et al. (2000)
6 more

18 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
17 more