› 1cjw

X-ray diffraction
1.8Å resolution

SEROTONIN N-ACETYLTRANSFERASE COMPLEXED WITH A BISUBSTRATE ANALOG

Released:
DOI: 10.2210/pdb1cjw/pdb
PDB entry 1cjw coloured by chain, front view.
PDB entry 1cjw coloured by chain, side view.
PDB entry 1cjw coloured by chain, top view.
Source organism: Ovis aries
Primary publication:
The structural basis of ordered substrate binding by serotonin N-acetyltransferase: enzyme complex at 1.8 A resolution with a bisubstrate analog.
Hickman AB, Namboodiri MA, Klein DC, Dyda F
Cell 97 361-9 (1999)
PMID: 10319816

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + a 2-arylethylamine = CoA + an N-acetyl-2-arylethylamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serotonin N-acetyltransferase Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.63 KDa
Source organism: Ovis aries
Expression system: Escherichia coli
UniProt:
  • Canonical: Q29495 (Residues: 30-195; Coverage: 80%)
Gene names: AANAT, SNAT
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand COT 1 x COT
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2221
Unit cell:
a: 53.19Å b: 68.98Å c: 89.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.23
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Histone acetyltransferases.
Roth et al. (2001)
16 more

19 mentions without citation

Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).
Salah Ud-Din et al. (2016)
18 more