› 1cl2

X-ray diffraction
2.2Å resolution

CYSTATHIONINE BETA-LYASE (CBL) FROM ESCHERICHIA COLI IN COMPLEX WITH AMINOETHOXYVINYLGLYCINE

Released:
DOI: 10.2210/pdb1cl2/pdb
PDB entry 1cl2 coloured by chain, front view.
PDB entry 1cl2 coloured by chain, side view.
PDB entry 1cl2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study.
Clausen T, Huber R, Messerschmidt A, Pohlenz HD, Laber B
Biochemistry 36 12633-43 (1997)
PMID: 9376370

Function and Biology Details

Reactions catalysed: 
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
 (1a) L-cysteine = 2-aminoprop-2-enoate + sulfide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-lyase MetC Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 43.26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06721 (Residues: 1-395; Coverage: 100%)
Gene names: JW2975, b3008, metC
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PPG 2 x PPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2221
Unit cell:
a: 61.1Å b: 154.7Å c: 152.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 not available
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
7 more

7 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
6 more