› 1cl8

X-ray diffraction
1.8Å resolution

A PRE-TRANSITION STATE ECO RI ENDONUCLEASE/COGNATE DNA (TCGCGAPTTCGCG) COMPLEX WITH DNA BASE ANALOG PURINE (P)

Released:
DOI: 10.2210/pdb1cl8/pdb
PDB entry 1cl8 coloured by chain, front view.
PDB entry 1cl8 coloured by chain, side view.
PDB entry 1cl8 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Horvath M, Rosenberg JM

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113546 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme EcoRI Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 30.97 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P00642 (Residues: 2-277; Coverage: 100%)
Gene name: ecoRIR
Sequence domains: Restriction endonuclease EcoRI
Structure domains: Eco RI Endonuclease, subunit A
DNA (5'-D(*TP*CP*GP*CP*GP*AP*(PRN)P*TP*TP*CP*GP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PRN 1 x PRN

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P321
Unit cell:
a: 117.833Å b: 117.833Å c: 48.989Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.243 0.287
Expression system: Not provided

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