› 1cm8

X-ray diffraction
2.4Å resolution

PHOSPHORYLATED MAP KINASE P38-GAMMA

Released:
DOI: 10.2210/pdb1cm8/pdb
PDB entry 1cm8 coloured by chain, front view.
PDB entry 1cm8 coloured by chain, side view.
PDB entry 1cm8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of phosphorylated p38gamma is monomeric and reveals a conserved activation-loop conformation.
Bellon S, Fitzgibbon MJ, Fox T, Hsiao HM, Wilson KP
Structure 7 1057-65 (1999)
PMID: 10508788

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 42.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53778 (Residues: 1-367; Coverage: 100%)
Gene names: ERK6, MAPK12, SAPK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ANP 2 x ANP
2 modified residues:
Ligand TPO 2 x TPO
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 63.5Å b: 66.82Å c: 206.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.283
Expression system: Escherichia coli

Quick links

Citations

15 review citations

GSK-3: tricks of the trade for a multi-tasking kinase.
Doble et al. (2003)
14 more

35 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
34 more