› 1cq7

X-ray diffraction
2.4Å resolution

ASPARTATE AMINOTRANSFERASE (E.C. 2.6.1.1) COMPLEXED WITH C5-PYRIDOXAL-5P-PHOSPHATE

Released:
DOI: 10.2210/pdb1cq7/pdb
PDB entry 1cq7 coloured by chain, front view.
PDB entry 1cq7 coloured by chain, side view.
PDB entry 1cq7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Free energy requirement for domain movement of an enzyme.
Ishijima J, Nakai T, Kawaguchi S, Hirotsu K, Kuramitsu S
J Biol Chem 275 18939-45 (2000)
PMID: 10858450

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00509 (Residues: 1-396; Coverage: 100%)
Gene names: JW0911, aspC, b0928
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PY5 1 x PY5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 157.58Å b: 85.59Å c: 78.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.251
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural Consideration of the Working Mechanism of Fold Type I Transaminases From Eubacteria: Overt and Covert Movement.
Kwon et al. (2019)
4 other articles

2 mentions without citation

Developing an effective polarizable bond method for small molecules with application to optimized molecular docking.
Duan et al. (2020)
1 more