› 1cqt

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT

Released:
DOI: 10.2210/pdb1cqt/pdb
PDB entry 1cqt coloured by chain, front view.
PDB entry 1cqt coloured by chain, side view.
PDB entry 1cqt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface.
Chasman D, Cepek K, Sharp PA, Pabo CO
Genes Dev 13 2650-7 (1999)
PMID: 10541551

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113554 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
POU domain, class 2, transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14859 (Residues: 277-439; Coverage: 22%)
Gene names: OCT1, OTF1, POU2F1
Sequence domains:
Structure domains:
POU domain class 2-associating factor 1 Chains: I, J
Molecule details ›
Chains: I, J
Length: 44 amino acids
Theoretical weight: 4.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16633 (Residues: 1-44; Coverage: 17%)
Gene names: BOB1, OBF1, POU2AF1
DNA (5'-D(*TP*GP*TP*AP*TP*GP*CP*AP*AP*AP*TP*AP*AP*GP*G)-3') Chains: M, O
Molecule details ›
Chains: M, O
Length: 15 nucleotides
Theoretical weight: 4.66 KDa
DNA (5'-D(*AP*CP*CP*TP*TP*AP*TP*TP*TP*GP*CP*AP*TP*AP*C)-3') Chains: N, P
Molecule details ›
Chains: N, P
Length: 15 nucleotides
Theoretical weight: 4.52 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P41212
Unit cell:
a: 93.722Å b: 93.722Å c: 152.651Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.264 0.326
Expression system: Escherichia coli

Quick links

Citations

16 review citations

The herpes simplex virus VP16-induced complex: the makings of a regulatory switch.
Wysocka et al. (2003)
15 more

8 mentions without citation

Homeodomain revisited: a lesson from disease-causing mutations.
Chi YI. (2005)
7 more