› 1crx

X-ray diffraction
2.4Å resolution

CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I

Released:
DOI: 10.2210/pdb1crx/pdb
PDB entry 1crx coloured by chain, front view.
PDB entry 1crx coloured by chain, side view.
PDB entry 1crx coloured by chain, top view.
Source organism: Punavirus P1
Primary publication:
Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse.
Guo F, Gopaul DN, van Duyne GD
Nature 389 40-6 (1997)
PMID: 9288963

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113556 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Recombinase cre Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 36.41 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06956 (Residues: 20-341; Coverage: 94%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
DNA (5'-D(*TP*AP*TP*AP*AP*CP*TP*TP*CP*GP*TP*AP*TP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Punavirus P1
Expression system: Not provided
DNA (5'-D(*AP*TP*AP*TP*GP*CP*TP*AP*TP*AP*CP*GP*AP*AP*GP*TP*TP*AP*T)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Punavirus P1
Expression system: Not provided
DNA (5'-D(P*AP*TP*AP*AP*CP*TP*TP*CP*GP*TP*AP*TP*AP*GP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 15 nucleotides
Theoretical weight: 4.57 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 107.7Å b: 121Å c: 180.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.263
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

48 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
47 more

24 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
23 more