› 1crx

X-ray diffraction
2.4Å resolution

CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I

Released:
DOI: 10.2210/pdb1crx/pdb
Model geometry
Fit model/data
PDB entry 1crx coloured by chain, front view.
PDB entry 1crx coloured by chain, side view.
PDB entry 1crx coloured by chain, top view.
Source organism: Punavirus P1
Primary publication:
Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse.
Guo F, Gopaul DN, van Duyne GD
Nature 389 40-6 (1997)
PMID: 9288963

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-290489 (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Recombinase cre Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 36.41 KDa
Source organism: Punavirus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06956 (Residues: 20-341; Coverage: 94%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
Recombinase cre Chain: B
Molecule details ›
Chain: B
Length: 322 amino acids
Theoretical weight: 36.49 KDa
Source organism: Punavirus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06956 (Residues: 20-341; Coverage: 94%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
DNA (5'-D(*TP*AP*TP*AP*AP*CP*TP*TP*CP*GP*TP*AP*TP*AP*G)-3') Chain: C
DNA (5'-D(*AP*TP*AP*TP*GP*CP*TP*AP*TP*AP*CP*GP*AP*AP*GP*TP*TP*AP*T)-3') Chains: D, F
DNA (5'-D(P*AP*TP*AP*AP*CP*TP*TP*CP*GP*TP*AP*TP*AP*GP*C)-3') Chain: E

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 107.7Å b: 121Å c: 180.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

52 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
51 more

25 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
24 more