› 1cs4

X-ray diffraction
2.5Å resolution

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE: COMPLEX WITH 2'-DEOXY-ADENOSINE 3'-MONOPHOSPHATE, PYROPHOSPHATE AND MG

Released:
DOI: 10.2210/pdb1cs4/pdb
PDB entry 1cs4 coloured by chain, front view.
PDB entry 1cs4 coloured by chain, side view.
PDB entry 1cs4 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for P-site inhibition of adenylyl cyclase.
Tesmer JJ, Dessauer CW, Sunahara RK, Murray LD, Johnson RA, Gilman AG, Sprang SR
Biochemistry 39 14464-71 (2000)
PMID: 11087399

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-138237 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Adenylate cyclase type 5 Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.53 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P30803 (Residues: 441-661; Coverage: 18%)
Gene name: ADCY5
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Adenylate cyclase type 2 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P26769 (Residues: 870-1081; Coverage: 19%)
Gene name: Adcy2
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: C
Molecule details ›
Chain: C
Length: 394 amino acids
Theoretical weight: 45.77 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04896 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand MG 2 x MG
Ligand FOK 1 x FOK
Ligand MES 2 x MES
Ligand POP 1 x POP
Ligand 101 1 x 101
Ligand CL 1 x CL
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21212
Unit cell:
a: 118.5Å b: 134.5Å c: 71.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.216 0.264
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Physiological roles for G protein-regulated adenylyl cyclase isoforms: insights from knockout and overexpression studies.
Sadana et al. (2009)
10 more

13 mentions without citation

International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases.
Dessauer et al. (2017)
12 more