› 1ctp

X-ray diffraction
2.9Å resolution

STRUCTURE OF THE MAMMALIAN CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE AND AN INHIBITOR PEPTIDE DISPLAYS AN OPEN CONFORMATION

Released:
DOI: 10.2210/pdb1ctp/pdb
PDB entry 1ctp coloured by chain, front view.
PDB entry 1ctp coloured by chain, side view.
PDB entry 1ctp coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the mammalian catalytic subunit of cAMP-dependent protein kinase and an inhibitor peptide displays an open conformation.
Karlsson R, Zheng J, Xuong N, Taylor SS, Sowadski JM
Acta Crystallogr D Biol Crystallogr 49 381-8 (1993)
PMID: 15299513

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153240 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.62 KDa
Source organism: Sus scrofa
Expression system: Not provided
UniProt:
  • Canonical: P36887 (Residues: 2-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.48 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61926 (Residues: 6-25; Coverage: 26%)
Gene names: PKIA, PRKACN1

Ligands and Environments

1 bound ligand:
Ligand MYR 1 x MYR
2 modified residues:
Ligand TPO 1 x TPO
Ligand TYI 1 x TYI

Experiments and Validation Details

Entry percentile scores
Spacegroup: P4132
Unit cell:
a: 171.5Å b: 171.5Å c: 171.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 not available not available
Expression system: Not provided

Quick links

Citations

6 review citations

Three protein kinase structures define a common motif.
Taylor et al. (1994)
5 more

18 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
17 more