› 1cv2

X-ray diffraction
1.58Å resolution

Hydrolytic haloalkane dehalogenase linb from sphingomonas paucimobilis UT26 AT 1.6 A resolution

Released:
DOI: 10.2210/pdb1cv2/pdb
PDB entry 1cv2 coloured by chain, front view.
PDB entry 1cv2 coloured by chain, side view.
PDB entry 1cv2 coloured by chain, top view.
Source organism: Sphingomonas paucimobilis
Primary publication:
Crystal structure of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26.
Marek J, Vévodová J, Smatanová IK, Nagata Y, Svensson LA, Newman J, Takagi M, Damborský J
Biochemistry 39 14082-6 (2000)
PMID: 11087355

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.14 KDa
Source organism: Sphingomonas paucimobilis
Expression system: Escherichia coli
UniProt:
  • Canonical: D4Z2G1 (Residues: 1-296; Coverage: 100%)
Gene names: SJA_C1-19590, linB
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21212
Unit cell:
a: 50.264Å b: 71.669Å c: 72.698Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 not available 0.211
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation.
Lal et al. (2010)
8 more

8 mentions without citation

CAVER: a new tool to explore routes from protein clefts, pockets and cavities.
Petrek et al. (2006)
7 more