› 1cwb

X-ray diffraction
2.2Å resolution

THE X-RAY STRUCTURE OF (MEBM2T)1-CYCLOSPORIN COMPLEXED WITH CYCLOPHILIN A PROVIDES AN EXPLANATION FOR ITS ANOMALOUSLY HIGH IMMUNOSUPPRESSIVE ACTIVITY

Released:
DOI: 10.2210/pdb1cwb/pdb
PDB entry 1cwb coloured by chain, front view.
PDB entry 1cwb coloured by chain, side view.
PDB entry 1cwb coloured by chain, top view.
Source organisms:
Primary publication:
The X-ray structure of (MeBm2t)1-cyclosporin complexed with cyclophilin A provides an explanation for its anomalously high immunosuppressive activity.
Mikol V, Kallen J, Walkinshaw MD
Protein Eng 7 597-603 (1994)
PMID: 8073029

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238754 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidyl-prolyl cis-trans isomerase A Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 18.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62937 (Residues: 1-165; Coverage: 100%)
Gene names: CYPA, PPIA
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like
CYCLOSPORIN A Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.23 KDa
Source organism: Tolypocladium inflatum
Expression system: Not provided

Ligands and Environments

No bound ligands
6 modified residues:
Ligand DAL 1 x DAL
Ligand MLE 4 x MLE
Ligand MVA 1 x MVA
Ligand DMT 1 x DMT
Ligand ABA 1 x ABA
Ligand SAR 1 x SAR

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 36.869Å b: 61.292Å c: 71.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.162 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Cyclophilins: proteins in search of function.
Kumari et al. (2013)
6 other articles