› 1cwp

X-ray diffraction
3.2Å resolution

STRUCTURES OF THE NATIVE AND SWOLLEN FORMS OF COWPEA CHLOROTIC MOTTLE VIRUS DETERMINED BY X-RAY CRYSTALLOGRAPHY AND CRYO-ELECTRON MICROSCOPY

Released:
DOI: 10.2210/pdb1cwp/pdb
PDB entry 1cwp coloured by chain, front view.
PDB entry 1cwp coloured by chain, side view.
PDB entry 1cwp coloured by chain, top view.
Source organism: Cowpea chlorotic mottle virus
Primary publication:
Structures of the native and swollen forms of cowpea chlorotic mottle virus determined by X-ray crystallography and cryo-electron microscopy.
Speir JA, Munshi S, Wang G, Baker TS, Johnson JE
Structure 3 63-78 (1995)
PMID: 7743132

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 360-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137126 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Capsid protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 190 amino acids
Theoretical weight: 20.34 KDa
Source organism: Cowpea chlorotic mottle virus
UniProt:
  • Canonical: P03601 (Residues: 1-190; Coverage: 100%)
Gene name: ORF3b
Sequence domains: Bromovirus coat protein
Structure domains: Satellite virus coat domain
RNA (5'-R(*AP*UP*AP*U)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 4 nucleotides
Theoretical weight: 1.23 KDa
Source organism: Cowpea chlorotic mottle virus
Expression system: Not provided
RNA (5'-R(*AP*U)-3') Chain: E
Molecule details ›
Chain: E
Length: 2 nucleotides
Theoretical weight: 590 Da
Source organism: Cowpea chlorotic mottle virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 381.3Å b: 381.3Å c: 408.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.31 0.31 not available
Expression system: Not provided

Quick links

Citations

64 review citations

Adding the third dimension to virus life cycles: three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs.
Baker et al. (1999)
63 more

42 mentions without citation

Caged protein nanoparticles for drug delivery.
Molino et al. (2014)
41 more