› 1cy0

X-ray diffraction
2.45Å resolution

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 3'-5'-ADENOSINE DIPHOSPHATE

Released:
DOI: 10.2210/pdb1cy0/pdb
PDB entry 1cy0 coloured by chain, front view.
PDB entry 1cy0 coloured by chain, side view.
PDB entry 1cy0 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Protein-nucleotide interactions in E. coli DNA topoisomerase I.
Feinberg H, Changela A, Mondragón A
Nat Struct Biol 6 961-8 (1999)
PMID: 10504732

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 67.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06612 (Residues: 1-597; Coverage: 69%)
Gene names: JW1266, b1274, supX, topA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand A3P 1 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 63.04Å b: 73.31Å c: 134.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.274
Expression system: Escherichia coli

Quick links

Citations

5 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
4 more

4 mentions without citation

The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member.
Allemand et al. (2005)
3 more