› 1cy1

X-ray diffraction
2.3Å resolution

COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH 5'PTPTPT

Released:
DOI: 10.2210/pdb1cy1/pdb
PDB entry 1cy1 coloured by chain, front view.
PDB entry 1cy1 coloured by chain, side view.
PDB entry 1cy1 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Protein-nucleotide interactions in E. coli DNA topoisomerase I.
Feinberg H, Changela A, Mondragón A
Nat Struct Biol 6 961-8 (1999)
PMID: 10504732

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chain: A
Molecule details ›
Chain: A
Length: 599 amino acids
Theoretical weight: 67.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06612 (Residues: 1-597; Coverage: 69%)
Gene names: JW1266, b1274, supX, topA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand TMP 2 x TMP
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 62.86Å b: 77.8Å c: 138.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.307
Expression system: Escherichia coli

Quick links

Citations

5 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
4 more

3 mentions without citation

New alkaloid antibiotics that target the DNA topoisomerase I of Streptococcus pneumoniae.
García et al. (2011)
2 more