› 1cy5

X-ray diffraction
1.3Å resolution

CRYSTAL STRUCTURE OF THE APAF-1 CARD

Released:
DOI: 10.2210/pdb1cy5/pdb
PDB entry 1cy5 coloured by chain, front view.
PDB entry 1cy5 coloured by chain, side view.
PDB entry 1cy5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of Apaf-1 caspase recruitment domain: an alpha-helical Greek key fold for apoptotic signaling.
Vaughn DE, Rodriguez J, Lazebnik Y, Joshua-Tor L
J Mol Biol 293 439-47 (1999)
PMID: 10543941

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptotic protease-activating factor 1 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 11.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14727 (Residues: 1-97; Coverage: 8%)
Gene names: APAF1, KIAA0413
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 36.35Å b: 46.91Å c: 54.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 not available 0.199
Expression system: Escherichia coli

Quick links

Citations

15 review citations

The mitochondrial death pathway and cardiac myocyte apoptosis.
Crow et al. (2004)
14 more

23 mentions without citation

Activation and assembly of the inflammasomes through conserved protein domain families.
Jin et al. (2015)
22 more