› 1cyy

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF THE 30 KDA FRAGMENT OF E. COLI DNA TOPOISOMERASE I. HEXAGONAL FORM

Released:
DOI: 10.2210/pdb1cyy/pdb
PDB entry 1cyy coloured by chain, front view.
PDB entry 1cyy coloured by chain, side view.
PDB entry 1cyy coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Conformational changes in E. coli DNA topoisomerase I.
Feinberg H, Lima CD, Mondragón A
Nat Struct Biol 6 918-22 (1999)
PMID: 10504724

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-139095 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 29.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06612 (Residues: 214-477; Coverage: 31%)
Gene names: JW1266, b1274, supX, topA
Sequence domains: DNA topoisomerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P6122
Unit cell:
a: 113.85Å b: 113.85Å c: 233.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.229 0.282
Expression system: Escherichia coli

Quick links

Citations

10 review citations

DNA topoisomerases: structure, function, and mechanism.
Champoux JJ. (2001)
9 more

1 mention without citation

Experimental and computational investigations of Ser10 and Lys13 in the binding and cleavage of DNA substrates by Escherichia coli DNA topoisomerase I.
Strahs et al. (2006)