› 1cza

X-ray diffraction
1.9Å resolution

MUTANT MONOMER OF RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE, GLUCOSE-6-PHOSPHATE, AND ADP

Released:
DOI: 10.2210/pdb1cza/pdb
PDB entry 1cza coloured by chain, front view.
PDB entry 1cza coloured by chain, side view.
PDB entry 1cza coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of mutant monomeric hexokinase I reveal multiple ADP binding sites and conformational changes relevant to allosteric regulation.
Aleshin AE, Kirby C, Liu X, Bourenkov GP, Bartunik HD, Fromm HJ, Honzatko RB
J Mol Biol 296 1001-15 (2000)
PMID: 10686099

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-1 Chain: N
Molecule details ›
Chain: N
Length: 917 amino acids
Theoretical weight: 102.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19367 (Residues: 1-917; Coverage: 100%)
Gene name: HK1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GLC 2 x GLC
Ligand G6P 2 x G6P
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21212
Unit cell:
a: 145.66Å b: 145.8Å c: 58.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.258
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
3 more

6 mentions without citation

Mechanisms underlying ubiquitin-driven selective mitochondrial and bacterial autophagy.
Goodall et al. (2022)
5 more