› 1d2t

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE

Released:
DOI: 10.2210/pdb1d2t/pdb
PDB entry 1d2t coloured by chain, front view.
PDB entry 1d2t coloured by chain, side view.
PDB entry 1d2t coloured by chain, top view.
Source organism: Shimwellia blattae
Primary publication:
X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdate.
Ishikawa K, Mihara Y, Gondoh K, Suzuki E, Asano Y
EMBO J 19 2412-23 (2000)
PMID: 10835340

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193376 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acid phosphatase Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 25.03 KDa
Source organism: Shimwellia blattae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S1A6 (Residues: 19-249; Coverage: 100%)
Gene name: pho
Sequence domains: PAP2 superfamily
Structure domains: Phosphatidic acid phosphatase type 2/haloperoxidase

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P6322
Unit cell:
a: 124.7Å b: 124.7Å c: 97.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.216 0.257
Expression system: Escherichia coli

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Citations

5 review citations

Deciphering the metabolism of undecaprenyl-phosphate: the bacterial cell-wall unit carrier at the membrane frontier.
Manat et al. (2014)
4 more

9 mentions without citation

Non-homologous isofunctional enzymes: a systematic analysis of alternative solutions in enzyme evolution.
Omelchenko et al. (2010)
8 more