› 1d6n

X-ray diffraction
2.7Å resolution

TERNARY COMPLEX STRUCTURE OF HUMAN HGPRTASE, PRPP, MG2+, AND THE INHIBITOR HPP REVEALS THE INVOLVEMENT OF THE FLEXIBLE LOOP IN SUBSTRATE BINDING

Released:
DOI: 10.2210/pdb1d6n/pdb
PDB entry 1d6n coloured by chain, front view.
PDB entry 1d6n coloured by chain, side view.
PDB entry 1d6n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ternary complex structure of human HGPRTase, PRPP, Mg2+, and the inhibitor HPP reveals the involvement of the flexible loop in substrate binding.
Balendiran GK, Molina JA, Xu Y, Torres-Martinez J, Stevens R, Focia PJ, Eakin AE, Sacchettini JC, Craig SP
Protein Sci 8 1023-31 (1999)
PMID: 10338013

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-132563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 24.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00492 (Residues: 5-218; Coverage: 98%)
Gene names: HPRT, HPRT1
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand PPO 2 x PPO
Ligand PRP 2 x PRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 129.56Å b: 65.85Å c: 51.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.278
Expression system: Escherichia coli

Quick links

Citations

29 citation in other articles

The nucleobase-ascorbate transporter (NAT) signature motif in UapA defines the function of the purine translocation pathway.
Koukaki et al. (2005)
28 more

3 mentions without citation

Database of traditional Chinese medicine and its application to studies of mechanism and to prescription validation.
Chen et al. (2006)
2 more