› 1dc1

X-ray diffraction
1.7Å resolution

RESTRICTION ENZYME BSOBI/DNA COMPLEX STRUCTURE: ENCIRCLEMENT OF THE DNA AND HISTIDINE-CATALYZED HYDROLYSIS WITHIN A CANONICAL RESTRICTION ENZYME FOLD

Released:
DOI: 10.2210/pdb1dc1/pdb
PDB entry 1dc1 coloured by chain, front view.
PDB entry 1dc1 coloured by chain, side view.
PDB entry 1dc1 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Restriction enzyme BsoBI-DNA complex: a tunnel for recognition of degenerate DNA sequences and potential histidine catalysis.
van der Woerd MJ, Pelletier JJ, Xu S, Friedman AM
Structure 9 133-44 (2001)
PMID: 11250198

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113666 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme BsoBI Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.74 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P70985 (Residues: 1-323; Coverage: 100%)
Gene name: bsoBIR
Sequence domains: Restriction endonuclease BsobI
Structure domains:
DNA (5'-D(*T*AP*TP*AP*CP*TP*CP*GP*AP*GP*TP*AP*T)-3') Chains: C, W
Molecule details ›
Chains: C, W
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand DIO 10 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 80.294Å b: 87.823Å c: 99.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.253
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
1 more

7 mentions without citation

An NMR approach to structural proteomics.
Yee et al. (2002)
6 more