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X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF NAD+-DEPENDENT DNA LIGASE FROM T. FILIFORMIS

Released:
DOI: 10.2210/pdb1dgs/pdb
PDB entry 1dgs coloured by chain, front view.
PDB entry 1dgs coloured by chain, side view.
PDB entry 1dgs coloured by chain, top view.
Source organism: Thermus filiformis
Primary publication:
Crystal structure of NAD(+)-dependent DNA ligase: modular architecture and functional implications.
Lee JY, Chang C, Song HK, Moon J, Yang JK, Kim HK, Kwon ST, Suh SW
EMBO J 19 1119-29 (2000)
PMID: 10698952

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 89.21Å b: 117.33Å c: 97.48Å
α: 90° β: 115.09° γ: 90°
R-values:
R R work R free
0.228 0.228 0.298

Quick links

Citations

18 review citations

Eukaryotic DNA ligases: structural and functional insights.
Ellenberger et al. (2008)
17 more

11 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
10 more