1dhy

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed:
Biphenyl-2,3-diol + O(2) = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
PDBe Complex ID:
PDB-CPX-148023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biphenyl-2,3-diol 1,2-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 32.15 KDa
Source organism: Pseudomonas sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P17297 (Residues: 2-293; Coverage: 100%)
Gene name: bphC
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I422
Unit cell:
a: 123Å b: 123Å c: 110.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.246
Expression system: Escherichia coli