› 1djo

X-ray diffraction
2Å resolution

Crystal structure of Pseudomonas 7A Glutaminase-asparaginase with the inhibitor donv covalently bound in the active site

Released:
DOI: 10.2210/pdb1djo/pdb
PDB entry 1djo coloured by chain, front view.
PDB entry 1djo coloured by chain, side view.
PDB entry 1djo coloured by chain, top view.
Source organism: Pseudomonas sp. 7A
Primary publication:
Reactions of Pseudomonas 7A glutaminase-asparaginase with diazo analogues of glutamine and asparagine result in unexpected covalent inhibitions and suggests an unusual catalytic triad Thr-Tyr-Glu.
Ortlund E, Lacount MW, Lewinski K, Lebioda L
Biochemistry 39 1199-204 (2000)
PMID: 10684596

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase-asparaginase Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 35.4 KDa
Source organism: Pseudomonas sp. 7A
UniProt:
  • Canonical: P10182 (Residues: 8-337; Coverage: 98%)
Gene name: ansB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CAB 2 x CAB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 78.37Å b: 135.89Å c: 137.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.214 0.238

Quick links

Citations

10 review citations

From one amino acid to another: tRNA-dependent amino acid biosynthesis.
Sheppard et al. (2008)
9 more

3 mentions without citation

Crystal structure of the catalytic domain of RluD, the only rRNA pseudouridine synthase required for normal growth of Escherichia coli.
Del Campo et al. (2004)
2 more