› 1dmq

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF MUTANT ENZYME Y32F OF KETOSTEROID ISOMERASE FROM PSEUDOMONAS PUTIDA BIOTYPE B

Released:
DOI: 10.2210/pdb1dmq/pdb
PDB entry 1dmq coloured by chain, front view.
PDB entry 1dmq coloured by chain, side view.
PDB entry 1dmq coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Contribution of the hydrogen-bond network involving a tyrosine triad in the active site to the structure and function of a highly proficient ketosteroid isomerase from Pseudomonas putida biotype B.
Kim DH, Jang DS, Nam GH, Choi G, Kim JS, Ha NC, Kim MS, Oh BH, Choi KY
Biochemistry 39 4581-9 (2000)
PMID: 10769113

Function and Biology Details

Reaction catalysed: 
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.53 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P07445 (Residues: 1-131; Coverage: 100%)
Gene name: ksi
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: C2221
Unit cell:
a: 36.37Å b: 95.74Å c: 74.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.234
Expression system: Escherichia coli

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Citations

1 review citation

Catalytic efficiency of enzymes: a theoretical analysis.
Hammes-Schiffer S. (2013)
21 other articles

1 mention without citation

Structural double-mutant cycle analysis of a hydrogen bond network in ketosteroid isomerase from Pseudomonas putida biotype B.
Jang et al. (2004)