› 1dmt

X-ray diffraction
2.1Å resolution

STRUCTURE OF HUMAN NEUTRAL ENDOPEPTIDASE COMPLEXED WITH PHOSPHORAMIDON

Released:
DOI: 10.2210/pdb1dmt/pdb
PDB entry 1dmt coloured by chain, front view.
PDB entry 1dmt coloured by chain, side view.
PDB entry 1dmt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human neutral endopeptidase (Neprilysin) complexed with phosphoramidon.
Oefner C, D'Arcy A, Hennig M, Winkler FK, Dale GE
J Mol Biol 296 341-9 (2000)
PMID: 10669592

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140129 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neprilysin Chain: A
Molecule details ›
Chain: A
Length: 696 amino acids
Theoretical weight: 79.53 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08473 (Residues: 55-750; Coverage: 93%)
Gene names: EPN, MME
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 3 x NAG
Ligand ZN 1 x ZN
Ligand RDF 1 x RDF
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P3221
Unit cell:
a: 107.58Å b: 107.58Å c: 112.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.242
Expression system: Saccharomyces cerevisiae

Quick links

Citations

29 review citations

The neprilysin (NEP) family of zinc metalloendopeptidases: genomics and function.
Turner et al. (2001)
28 more

23 mentions without citation

Amyloid beta-degrading cryptidases: insulin degrading enzyme, presequence peptidase, and neprilysin.
Malito et al. (2008)
22 more