› 1dp7

X-ray diffraction
1.5Å resolution

COCRYSTAL STRUCTURE OF RFX-DBD IN COMPLEX WITH ITS COGNATE X-BOX BINDING SITE

Released:
DOI: 10.2210/pdb1dp7/pdb
PDB entry 1dp7 coloured by chain, front view.
PDB entry 1dp7 coloured by chain, side view.
PDB entry 1dp7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the winged-helix protein hRFX1 reveals a new mode of DNA binding.
Gajiwala KS, Chen H, Cornille F, Roques BP, Reith W, Mach B, Burley SK
Nature 403 916-21 (2000)
PMID: 10706293

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113702 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
MHC class II regulatory factor RFX1 Chain: P
Molecule details ›
Chain: P
Length: 76 amino acids
Theoretical weight: 8.83 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P22670 (Residues: 438-513; Coverage: 8%)
Gene name: RFX1
Sequence domains: RFX DNA-binding domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*CP*GP*(BRU)P*TP*AP*CP*CP*AP*(BRU)P*GP*GP*TP*AP*AP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 5.03 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand PEG 1 x PEG
1 modified residue:
Ligand BRU 2 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2, NSLS BEAMLINE X9A
Spacegroup: C2
Unit cell:
a: 72.843Å b: 42.752Å c: 52.277Å
α: 90° β: 109.21° γ: 90°
R-values:
R R work R free
0.192 0.192 0.228
Expression system: Not provided

Quick links

Citations

29 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
28 more

22 mentions without citation

Structural mechanisms of human RecQ helicases WRN and BLM.
Kitano K. (2014)
21 more