› 1drg

X-ray diffraction
2.55Å resolution

CRYSTAL STRUCTURE OF TRIMERIC CRE RECOMBINASE-LOX COMPLEX

Released:
DOI: 10.2210/pdb1drg/pdb
PDB entry 1drg coloured by chain, front view.
PDB entry 1drg coloured by chain, side view.
PDB entry 1drg coloured by chain, top view.
Source organism: Punavirus P1
Primary publication:
Quasi-equivalence in site-specific recombinase structure and function: crystal structure and activity of trimeric Cre recombinase bound to a three-way Lox DNA junction.
Woods KC, Martin SS, Chu VC, Baldwin EP
J Mol Biol 313 49-69 (2001)
PMID: 11601846

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113705 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Recombinase cre Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.47 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli
UniProt:
  • Canonical: P06956 (Residues: 21-343; Coverage: 94%)
Gene name: cre
Sequence domains: Phage integrase family
Structure domains:
5'-D(*TP*AP*TP*AP*AP*CP*TP*TP*CP*GP*TP*AP*TP*AP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Punavirus P1
Expression system: Not provided
5'-D(*AP*TP*AP*TP*GP*CP*TP*AP*TP*AP*CP*GP*AP*AP*GP*TP*TP*AP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I23
Unit cell:
a: 160.99Å b: 160.99Å c: 160.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Mechanisms of site-specific recombination.
Grindley et al. (2006)
4 more

2 mentions without citation

Supramolecular DNA Three-Way Junction Motifs With a Bridging Metal Center.
Takezawa et al. (2019)
1 more