› 1du0

X-ray diffraction
2Å resolution

ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX

Released:
DOI: 10.2210/pdb1du0/pdb
PDB entry 1du0 coloured by chain, front view.
PDB entry 1du0 coloured by chain, side view.
PDB entry 1du0 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Exploring the role of glutamine 50 in the homeodomain-DNA interface: crystal structure of engrailed (Gln50 --> ala) complex at 2.0 A.
Grant RA, Rould MA, Klemm JD, Pabo CO
Biochemistry 39 8187-92 (2000)
PMID: 10889025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113713 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Segmentation polarity homeobox protein engrailed Chains: A, B
Molecule details ›
Chains: A, B
Length: 57 amino acids
Theoretical weight: 6.91 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P02836 (Residues: 456-512; Coverage: 10%)
Gene names: CG9015, en
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*TP*TP*TP*TP*GP*CP*CP*AP*TP*GP*TP*AP*AP*TP*TP*AP*CP*CP*TP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*AP*TP*TP*AP*GP*GP*TP*AP*AP*TP*TP*AP*CP*AP*TP*GP*GP*CP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 126.9Å b: 45.55Å c: 71.62Å
α: 90° β: 119.9° γ: 90°
R-values:
R R work R free
0.237 0.234 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
2 more

13 mentions without citation

Low-Affinity Binding Sites and the Transcription Factor Specificity Paradox in Eukaryotes.
Kribelbauer et al. (2019)
12 more