› 1dul

X-ray diffraction
1.8Å resolution

STRUCTURE OF THE RIBONUCLEOPROTEIN CORE OF THE E. COLI SIGNAL RECOGNITION PARTICLE

Released:
DOI: 10.2210/pdb1dul/pdb
PDB entry 1dul coloured by chain, front view.
PDB entry 1dul coloured by chain, side view.
PDB entry 1dul coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the ribonucleoprotein core of the signal recognition particle.
Batey RT, Rambo RP, Lucast L, Rha B, Doudna JA
Science 287 1232-9 (2000)
PMID: 10678824

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142810 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 12.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGD7 (Residues: 328-432; Coverage: 23%)
Gene names: JW5414, b2610, ffh
Sequence domains: Signal peptide binding domain
Structure domains: Signal recognition particle, SRP54 subunit, M-domain
4.5 S RNA DOMAIN IV Chain: B
Molecule details ›
Chain: B
Length: 49 nucleotides
Theoretical weight: 15.93 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand K 3 x K
Ligand MG 4 x MG
2 modified residues:
Ligand CCC 1 x CCC
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 136.567Å b: 78.291Å c: 32.849Å
α: 90° β: 96.14° γ: 90°
R-values:
R R work R free
0.199 0.199 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

50 review citations

Type V protein secretion pathway: the autotransporter story.
Henderson et al. (2004)
49 more

14 mentions without citation

Molecular mechanism of co-translational protein targeting by the signal recognition particle.
Saraogi et al. (2011)
13 more