› 1dw9

X-ray diffraction
1.65Å resolution

Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site

Released:
DOI: 10.2210/pdb1dw9/pdb
PDB entry 1dw9 coloured by chain, front view.
PDB entry 1dw9 coloured by chain, side view.
PDB entry 1dw9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site.
Walsh MA, Otwinowski Z, Perrakis A, Anderson PM, Joachimiak A
Structure 8 505-14 (2000)
PMID: 10801492

Function and Biology Details

Reaction catalysed: 
(1a) cyanate + HCO(3)(-) + H(+) = carbamate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanate hydratase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 156 amino acids
Theoretical weight: 17.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00816 (Residues: 1-156; Coverage: 100%)
Gene names: JW0331, b0340, cnt, cynS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 10 x CL
Ligand SO4 23 x SO4
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 76.34Å b: 81.03Å c: 82.3Å
α: 70.3° β: 72.2° γ: 66.4°
R-values:
R R work R free
0.15 0.15 0.189
Expression system: Escherichia coli

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Citations

21 citation in other articles

The genome of the ammonia-oxidizing Candidatus Nitrososphaera gargensis: insights into metabolic versatility and environmental adaptations.
Spang et al. (2012)
20 more

13 mentions without citation

Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard.
Terwilliger et al. (2009)
12 more