› 1dz5

Solution NMR

The NMR structure of the 38KDa U1A protein-PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein

Released:
DOI: 10.2210/pdb1dz5/pdb
PDB entry 1dz5 coloured by chain, ensemble of 13 models, front view.
PDB entry 1dz5 coloured by chain, ensemble of 13 models, side view.
PDB entry 1dz5 coloured by chain, ensemble of 13 models, top view.
Source organism: Homo sapiens
Primary publication:
The NMR structure of the 38 kDa U1A protein - PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein.
Varani L, Gunderson SI, Mattaj IW, Kay LE, Neuhaus D, Varani G
Nat Struct Biol 7 329-35 (2000)
PMID: 10742179

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140519 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 2-102; Coverage: 36%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
PIE, RNA (5'-R(*GP*AP*GP*AP*CP*AP*UP*UP*GP*CP*AP*CP*CP* CP*GP*GP*AP*GP*UP*CP*UP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 7.03 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: RESTRAINED MOLECULAR DYNAMICS
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
20 more

4 mentions without citation

RNA sequence- and shape-dependent recognition by proteins in the ribonucleoprotein particle.
Stefl et al. (2005)
3 more