› 1dzf

X-ray diffraction
1.9Å resolution

RPB5 from S.cerevisiae

Released:
DOI: 10.2210/pdb1dzf/pdb
PDB entry 1dzf coloured by chain, front view.
PDB entry 1dzf coloured by chain, side view.
PDB entry 1dzf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target.
Todone F, Weinzierl RO, Brick P, Onesti S
Proc Natl Acad Sci U S A 97 6306-10 (2000)
PMID: 10841537

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 25.12 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P20434 (Residues: 1-215; Coverage: 100%)
Gene names: RPA7, RPB5, RPC9, YBR1204, YBR154C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2221
Unit cell:
a: 45.05Å b: 82.22Å c: 134.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.271
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure of eukaryotic RNA polymerases.
Cramer et al. (2008)
2 more

9 mentions without citation

Rpb5, a subunit shared by eukaryotic RNA polymerases, cooperates with prefoldin-like Bud27/URI.
Martínez-Fernández et al. (2018)
8 more