› 1e4i

X-ray diffraction
2Å resolution

2-deoxy-2-fluoro-beta-D-glucosyl/enzyme intermediate complex of the beta-glucosidase from Bacillus polymyxa

Released:
DOI: 10.2210/pdb1e4i/pdb
PDB entry 1e4i coloured by chain, front view.
PDB entry 1e4i coloured by chain, side view.
PDB entry 1e4i coloured by chain, top view.
Source organism: Paenibacillus polymyxa
Primary publication:
Structural basis of increased resistance to thermal denaturation induced by single amino acid substitution in the sequence of beta-glucosidase A from Bacillus polymyxa.
Sanz-Aparicio J, Hermoso JA, Martínez-Ripoll M, González B, López-Camacho C, Polaina J
Proteins 33 567-76 (1998)
PMID: 9849940

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase A Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 51.57 KDa
Source organism: Paenibacillus polymyxa
Expression system: Escherichia coli
UniProt:
  • Canonical: P22073 (Residues: 2-448; Coverage: 100%)
Gene name: bglA
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand G2F 1 x G2F
Ligand NFG 1 x NFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: I422
Unit cell:
a: 136.23Å b: 136.23Å c: 173.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.25
Expression system: Escherichia coli

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Citations

1 review citation

Directed evolution of (betaalpha)(8)-barrel enzymes.
Höcker B. (2005)
11 other articles

9 mentions without citation

Oligomerization as a strategy for cold adaptation: Structure and dynamics of the GH1 β-glucosidase from Exiguobacterium antarcticum B7.
Zanphorlin et al. (2016)
8 more