› 1e6g

X-ray diffraction
2.3Å resolution

A-SPECTRIN SH3 DOMAIN A11V, V23L, M25I, V53I, V58L MUTANT

Released:
DOI: 10.2210/pdb1e6g/pdb
PDB entry 1e6g coloured by chain, front view.
PDB entry 1e6g coloured by chain, side view.
PDB entry 1e6g coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Conformational strain in the hydrophobic core and its implications for protein folding and design.
Ventura S, Vega MC, Lacroix E, Angrand I, Spagnolo L, Serrano L
Nat Struct Biol 9 485-93 (2002)
PMID: 12006985

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.28 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07751 (Residues: 965-1025; Coverage: 3%)
Gene names: SPTA2, SPTAN1
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18X
Spacegroup: P212121
Unit cell:
a: 29.19Å b: 43.52Å c: 53.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.267 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Rational engineering of enzyme stability.
Eijsink et al. (2004)
11 more

1 mention without citation

High-resolution structure of an alpha-spectrin SH3-domain mutant with a redesigned hydrophobic core.
Cámara-Artigas et al. (2010)