› 1e77

X-ray diffraction
2.69Å resolution

COMPLEX OF ACTIVE MUTANT (Q365->C) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES WITH SUBSTRATE

Released:
DOI: 10.2210/pdb1e77/pdb
PDB entry 1e77 coloured by chain, front view.
PDB entry 1e77 coloured by chain, side view.
PDB entry 1e77 coloured by chain, top view.
Source organism: Leuconostoc mesenteroides
Primary publication:
An examination of the role of asp-177 in the His-Asp catalytic dyad of Leuconostoc mesenteroides glucose 6-phosphate dehydrogenase: X-ray structure and pH dependence of kinetic parameters of the D177N mutant enzyme.
Cosgrove MS, Gover S, Naylor CE, Vandeputte-Rutten L, Adams MJ, Levy HR
Biochemistry 39 15002-11 (2000)
PMID: 11106478

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NAD(P)(+) = 6-phospho-D-glucono-1,5-lactone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 54.34 KDa
Source organism: Leuconostoc mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: P11411 (Residues: 2-486; Coverage: 100%)
Gene name: zwf
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BG6 1 x BG6
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2
Unit cell:
a: 129.8Å b: 44.3Å c: 91.2Å
α: 90° β: 105.1° γ: 90°
R-values:
R R work R free
0.18 0.18 0.285
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
19 other articles

1 mention without citation

Identification and In Silico Characterization of Novel Helicobacter pylori Glucose-6-Phosphate Dehydrogenase Inhibitors.
Hernández-Ochoa et al. (2021)