› 1ebf

X-ray diffraction
2.3Å resolution

HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+

Released:
DOI: 10.2210/pdb1ebf/pdb
PDB entry 1ebf coloured by chain, front view.
PDB entry 1ebf coloured by chain, side view.
PDB entry 1ebf coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases.
DeLaBarre B, Thompson PR, Wright GD, Berghuis AM
Nat Struct Biol 7 238-44 (2000)
PMID: 10700284

Function and Biology Details

Reaction catalysed: 
L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 38.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P31116 (Residues: 2-359; Coverage: 100%)
Gene names: HOM6, J2132, YJR139C
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 80.396Å b: 80.396Å c: 250.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.218 0.269
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
2 more

6 mentions without citation

Evolution and multifarious horizontal transfer of an alternative biosynthetic pathway for the alternative polyamine sym-homospermidine.
Shaw et al. (2010)
5 more