› 1efa

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF

Released:
DOI: 10.2210/pdb1efa/pdb
PDB entry 1efa coloured by chain, front view.
PDB entry 1efa coloured by chain, side view.
PDB entry 1efa coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A closer view of the conformation of the Lac repressor bound to operator.
Bell CE, Lewis M
Nat Struct Biol 7 209-14 (2000)
PMID: 10700279

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-113732 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Lactose operon repressor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 333 amino acids
Theoretical weight: 35.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-333; Coverage: 93%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains:
DNA (5'-D(*GP*AP*AP*T*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NPF 3 x NPF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: R32
Unit cell:
a: 251.438Å b: 251.438Å c: 204.791Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.247 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Prokaryotic transcription regulators: more than just the helix-turn-helix motif.
Huffman et al. (2002)
12 more

69 mentions without citation

Predicting deleterious amino acid substitutions.
Ng et al. (2001)
68 more