› 1efl

X-ray diffraction
2.6Å resolution

HUMAN MALIC ENZYME IN A QUATERNARY COMPLEX WITH NAD, MG, AND TARTRONATE

Released:
DOI: 10.2210/pdb1efl/pdb
PDB entry 1efl coloured by chain, front view.
PDB entry 1efl coloured by chain, side view.
PDB entry 1efl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a closed form of human malic enzyme and implications for catalytic mechanism.
Yang Z, Floyd DL, Loeber G, Tong L
Nat Struct Biol 7 251-7 (2000)
PMID: 10700286

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = pyruvate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149937 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent malic enzyme, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 584 amino acids
Theoretical weight: 66.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23368 (Residues: 1-584; Coverage: 100%)
Gene name: ME2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
2 bound ligands:
Ligand MG 4 x MG
Ligand TTN 4 x TTN
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 228.8Å b: 117Å c: 114.3Å
α: 90° β: 109.2° γ: 90°
R-values:
R R work R free
0.206 0.206 0.285
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Malo-ethanolic fermentation in Saccharomyces and Schizosaccharomyces.
Volschenk et al. (2003)
47 other articles

3 mentions without citation

Three perspectives on the molecular basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.
Spudich JA. (2019)
2 more