› 1eg0

Electron Microscopy
11.5Å resolution

FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME

Released:
DOI: 10.2210/pdb1eg0/pdb
PDB entry 1eg0 coloured by chain, front view.
PDB entry 1eg0 coloured by chain, side view.
PDB entry 1eg0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure of the E. coli 70S ribosome at 11.5 A resolution.
Gabashvili IS, Agrawal RK, Spahn CM, Grassucci RA, Svergun DI, Frank J, Penczek P
Cell 100 537-49 (2000)
PMID: 10721991
Related structures: EMD-1003

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-143901 (preferred)
Entry contents:
11 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (15 distinct):
Small ribosomal subunit protein uS4 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.62 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P21466 (Residues: 43-200; Coverage: 79%)
Gene names: BSU29660, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: B
Molecule details ›
Chain: B
Length: 148 amino acids
Theoretical weight: 15.69 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P21467 (Residues: 1-148; Coverage: 89%)
Gene names: BSU01330, rpsE, spcA
Sequence domains:
Small ribosomal subunit protein bS6 Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 11.62 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q5SLP8 (Residues: 1-97; Coverage: 96%)
Gene names: TTHA0245, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: D
Molecule details ›
Chain: D
Length: 146 amino acids
Theoretical weight: 16.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P17291 (Residues: 1-146; Coverage: 94%)
Gene names: TTHA1696, rps7, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: E
Molecule details ›
Chain: E
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS15 Chain: F
Molecule details ›
Chain: F
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein uS17 Chain: G
Molecule details ›
Chain: G
Length: 93 amino acids
Theoretical weight: 10.57 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P23828 (Residues: 2-85; Coverage: 97%)
Gene name: rpsQ
Sequence domains: Ribosomal protein S17
PROTEIN (S20 RIBOSOMAL PROTEIN) Chain: H
Molecule details ›
Chain: H
Length: 100 amino acids
Theoretical weight: 8.53 KDa
Source organism: Escherichia coli
Large ribosomal subunit protein uL1 Chain: N
Molecule details ›
Chain: N
Length: 229 amino acids
Theoretical weight: 24.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q5SLP7 (Residues: 1-229; Coverage: 100%)
Gene names: TTHA0246, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein uL6 Chain: J
Molecule details ›
Chain: J
Length: 171 amino acids
Theoretical weight: 18.6 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q5L408 (Residues: 1-171; Coverage: 96%)
Gene names: GK0121, rplF
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein uL11 Chain: K
Molecule details ›
Chain: K
Length: 140 amino acids
Theoretical weight: 15 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P29395 (Residues: 1-140; Coverage: 99%)
Gene names: TM_0454, rplK
Sequence domains:
FRAGMENT OF 16S RRNA HELIX 23 Chain: I
Molecule details ›
Chain: I
Length: 33 nucleotides
Theoretical weight: 6.43 KDa
FRAGMENT OF 23S RRNA Chain: L
Molecule details ›
Chain: L
Length: 57 nucleotides
Theoretical weight: 18.35 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
HELIX 95 OF 23S RRNA Chain: M
Molecule details ›
Chain: M
Length: 26 nucleotides
Theoretical weight: 8.44 KDa
FORMYL-METHIONYL-TRNA Chain: O
Molecule details ›
Chain: O
Length: 76 nucleotides
Theoretical weight: 24.53 KDa
Sequence domains: tRNA

Ligands and Environments

No bound ligands
5 modified residues:
Ligand 4SU 1 x 4SU
Ligand H2U 1 x H2U
Ligand OMC 1 x OMC
Ligand 5MU 1 x 5MU
Ligand PSU 1 x PSU

Experiments and Validation Details

Entry percentile scores
Resolution: 11.5Å
Relevant EMDB volumes: EMD-1003

Quick links

Citations

48 review citations

How cryo-EM is revolutionizing structural biology.
Bai et al. (2015)
47 more

2 mentions without citation

Evolving data standards for cryo-EM structures.
Lawson et al. (2020)
1 more