› 1egj

X-ray diffraction
2.8Å resolution

DOMAIN 4 OF THE BETA COMMON CHAIN IN COMPLEX WITH AN ANTIBODY

Released:
DOI: 10.2210/pdb1egj/pdb
PDB entry 1egj coloured by chain, front view.
PDB entry 1egj coloured by chain, side view.
PDB entry 1egj coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor common beta-chain bound to an antagonist.
Rossjohn J, McKinstry WJ, Woodcock JM, McClure BJ, Hercus TR, Parker MW, Lopez AF, Bagley CJ
Blood 95 2491-8 (2000)
PMID: 10753826

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134481 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytokine receptor common subunit beta Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P32927 (Residues: 338-438; Coverage: 12%)
Gene names: CSF2RB, IL3RB, IL5RB
Structure domains: Immunoglobulins
Ig kappa chain V-III region MOPC 63 Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.59 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01661 (Residues: 21-131; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig gamma-2A chain C region, membrane-bound form Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-99; Coverage: 25%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P41212
Unit cell:
a: 77.61Å b: 77.61Å c: 296.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.228 0.288
Expression system: Escherichia coli

Quick links

Citations

20 review citations

The granulocyte-macrophage colony-stimulating factor receptor: linking its structure to cell signaling and its role in disease.
Hercus et al. (2009)
19 more

15 mentions without citation

An accurate, residue-level, pair potential of mean force for folding and binding based on the distance-scaled, ideal-gas reference state.
Zhang et al. (2004)
14 more