› 1ehi

X-ray diffraction
2.38Å resolution

D-ALANINE:D-LACTATE LIGASE (LMDDL2) OF VANCOMYCIN-RESISTANT LEUCONOSTOC MESENTEROIDES

Released:
DOI: 10.2210/pdb1ehi/pdb
PDB entry 1ehi coloured by chain, front view.
PDB entry 1ehi coloured by chain, side view.
PDB entry 1ehi coloured by chain, top view.
Source organism: Leuconostoc mesenteroides
Primary publication:
Enzymes of vancomycin resistance: the structure of D-alanine-D-lactate ligase of naturally resistant Leuconostoc mesenteroides.
Kuzin AP, Sun T, Jorczak-Baillass J, Healy VL, Walsh CT, Knox JR
Structure 8 463-70 (2000)
PMID: 10801495

Function and Biology Details

Reaction catalysed: 
ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanine--D-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 41.87 KDa
Source organism: Leuconostoc mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03ZI1 (Residues: 1-377; Coverage: 100%)
Gene names: LEUM_0264, ddl
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ADP 1 x ADP
Ligand PHY 1 x PHY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 118.2Å b: 90.8Å c: 82.1Å
α: 90° β: 92.1° γ: 90°
R-values:
R R work R free
0.184 0.184 0.257
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
5 more

9 mentions without citation

Allosteric inhibition of Staphylococcus aureus D-alanine:D-alanine ligase revealed by crystallographic studies.
Liu et al. (2006)
8 more