› 1eik

Solution NMR

Solution Structure of RNA Polymerase Subunit RPB5 from Methanobacterium Thermoautotrophicum

Released:
DOI: 10.2210/pdb1eik/pdb
PDB entry 1eik coloured by chain, ensemble of 10 models, front view.
PDB entry 1eik coloured by chain, ensemble of 10 models, side view.
PDB entry 1eik coloured by chain, ensemble of 10 models, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Solution structure of the RNA polymerase subunit RPB5 from Methanobacterium thermoautotrophicum.
Yee A, Booth V, Dharamsi A, Engel A, Edwards AM, Arrowsmith CH
Proc Natl Acad Sci U S A 97 6311-5 (2000)
PMID: 10841538

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit Rpo5 Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 8.83 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O27122 (Residues: 1-77; Coverage: 100%)
Gene names: MTH_1048, rpo5, rpoH
Sequence domains: RNA polymerase Rpb5, C-terminal domain
Structure domains: RPB5-like RNA polymerase subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry simulated annealing
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Determination of protein function, evolution and interactions by structural genomics.
Teichmann et al. (2001)
2 more

2 mentions without citation

Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum.
Mackereth et al. (2000)
1 more