› 1ej3

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF AEQUORIN

Released:
DOI: 10.2210/pdb1ej3/pdb
PDB entry 1ej3 coloured by chain, front view.
PDB entry 1ej3 coloured by chain, side view.
PDB entry 1ej3 coloured by chain, top view.
Source organism: Aequorea forskalea
Primary publication:
The crystal structure of the photoprotein aequorin at 2.3 A resolution.
Head JF, Inouye S, Teranishi K, Shimomura O
Nature 405 372-6 (2000)
PMID: 10830969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aequorin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.66 KDa
Source organism: Aequorea forskalea
Expression system: Escherichia coli
UniProt:
  • Canonical: P02592 (Residues: 6-196; Coverage: 97%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CZH 2 x CZH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P43212
Unit cell:
a: 81.27Å b: 81.27Å c: 163.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.253
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Imaging calcium in neurons.
Grienberger et al. (2012)
21 more

15 mentions without citation

Engineering bioluminescent proteins: expanding their analytical potential.
Rowe et al. (2009)
14 more