› 1ej5

Solution NMR

SOLUTION STRUCTURE OF THE AUTOINHIBITED CONFORMATION OF WASP

Released:
DOI: 10.2210/pdb1ej5/pdb
PDB entry 1ej5 coloured by chain, ensemble of 20 models, front view.
PDB entry 1ej5 coloured by chain, ensemble of 20 models, side view.
PDB entry 1ej5 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein.
Kim AS, Kakalis LT, Abdul-Manan N, Liu GA, Rosen MK
Nature 404 151-8 (2000)
PMID: 10724160

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin nucleation-promoting factor WAS Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42768 (Residues: 242-310; Coverage: 14%)
Gene names: IMD2, WAS
Sequence domains: P21-Rho-binding domain
Structure domains: CRIB domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

117 review citations

Cellular motility driven by assembly and disassembly of actin filaments.
Pollard et al. (2003)
116 more

14 mentions without citation

WASP family proteins: their evolution and its physiological implications.
Veltman et al. (2010)
13 more