› 1el4

X-ray diffraction
1.73Å resolution

STRUCTURE OF THE CALCIUM-REGULATED PHOTOPROTEIN OBELIN DETERMINED BY SULFUR SAS

Released:
DOI: 10.2210/pdb1el4/pdb
PDB entry 1el4 coloured by chain, front view.
PDB entry 1el4 coloured by chain, side view.
PDB entry 1el4 coloured by chain, top view.
Source organism: Obelia longissima
Primary publication:
Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure.
Liu ZJ, Vysotski ES, Chen CJ, Rose JP, Lee J, Wang BC
Protein Sci 9 2085-93 (2000)
PMID: 11152120

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Obelin Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.25 KDa
Source organism: Obelia longissima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27709 (Residues: 1-195; Coverage: 100%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand CTZ 1 x CTZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P62
Unit cell:
a: 81.562Å b: 81.562Å c: 86.088Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.188 0.205
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Bioluminescence in the sea.
Haddock et al. (2010)
10 more

8 mentions without citation

Crystal structure of obelin after Ca2+-triggered bioluminescence suggests neutral coelenteramide as the primary excited state.
Liu et al. (2006)
7 more