› 1emb

X-ray diffraction
2.13Å resolution

GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA, GLN 80 REPLACED WITH ARG

Released:
DOI: 10.2210/pdb1emb/pdb
PDB entry 1emb coloured by chain, front view.
PDB entry 1emb coloured by chain, side view.
PDB entry 1emb coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein.
Brejc K, Sixma TK, Kitts PA, Kain SR, Tsien RY, Ormö M, Remington SJ
Proc Natl Acad Sci U S A 94 2306-11 (1997)
PMID: 9122190

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.95 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 51.9Å b: 63.2Å c: 68.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

49 review citations

The green fluorescent protein.
Tsien RY. (1998)
48 more

24 mentions without citation

Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein.
Brejc et al. (1997)
23 more